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. 2021 Dec 8;9:792111. doi: 10.3389/fbioe.2021.792111

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Copyright © 2021 Wei, Wang, Xiang, Jiang, Gong, Su, Al Azad, Dong, Feng, Wu, Chan, Yang and Shi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of high-affinity GFP-specific and iRFP713-specific vNARs from immunized library. (A) Enrichment of GFP-specific vNARs during panning. (B) SDS-PAGE of seven purified anti-GFP vNARs. (C) Functional validation of vNARs. (D) EC₅₀ of vNARs against GFP. (E) SPR sensorgram of BsG3 binding with GFP. (F) Binding affinities and EC₅₀ of seven vNARs against GFP. The values represent mean ± standard error. (G) Enrichment of iRFP713-specific vNARs during panning. (H) SDS-PAGE of three purified anti-iRFP713 vNARs. (I) Binding affinities of three vNARs against iRFP713. The values represent mean ± standard error.