Figure 4.

Flow cytometry analysis of the immune microenvironment in MC38 tumor tissue, tumor-draining lymph node (TDLN), and spleen. Representative images of flow cytometry of (A) CD8⁺ T cells (gated on CD45⁺ cells), (C) CD69⁺ T cells (gated on CD3⁺CD8⁺ cells), (E) CD11c⁺MHCII⁺ cells (gated on CD45⁺ cells), and (G) CD11c⁺CD86⁺ cells (gated on CD45⁺ cells). Quantitative analysis of the proportion of (B) CD8⁺ cells in CD45⁺ cells, (D) CD3⁺CD8⁺CD69⁺ cells in lymphocytes, (F) CD11c⁺MHCII⁺ cells, and (H) CD11c⁺CD86⁺ cells in tumors. Representative images of flow cytometry of (I) CD3⁺CD8⁺ T cells (gated on CD45⁺ cells) and quantitative analysis of the proportion of (J) CD3⁺CD8⁺ T cells and (K) CD11c⁺MHCII⁺ cells in the TDLN. Representative images of flow cytometry of (L) CD8⁺ T cells (gated on CD45⁺CD3⁺ cells) and quantitative analysis of the proportion of (M) CD3⁺CD8⁺ T cells and (N) CD11c⁺MHCII⁺ cells in the spleen. Representative images of flow cytometry of (O) the PD-L1 level (gated on CD45^- cells) and quantitative analysis of PD-L1 mean fluorescence intensity (MFI) in tumors.