Figure 6.

HDAC6 inhibition with ACY-1083 reverses doxorubicin-induced reductions in microglial ramification in the hippocampus. (A) Filament length is defined as the sum of the length of each projection from the soma of a microglia. (B) Full branch level is the sum of the average branch level of each projection from the soma of a microglia. Branch level is a numerical value that begins at the beginning of a projection at the soma with a value of 1. At each branching point, the filament segment with the smaller diameter sequentially increases branch level while the dendrite with a larger diameter maintains the same branch level. (C) Schematic illustration visualizing increasing branch levels. The green dot represents the soma. (D) Sholl analysis quantifies the number of projection intersections per concentric spherical shell beginning at the soma and distanced at a radius of 1 μm apart. (E) Quantification of the area under the Sholl curve from D. Results are expressed as mean ± SEM; n = 5-10 mice/group; Two-way ANOVA with Tukey's post hoc analysis *p ≤ 0.05; **p ≤ 0.01; *** p ≤ 0.001.