Figure 4.

Trained immunity promotes macrophages to phagocytize bacteria. Mice and Raw264.7 cells were treated as described in Materials and methods section. After treatment, macrophages were obtained and rest overnight before co-cultured with pHrodo labeled E. coli^a for 1 h. Phagocytosis of (A) peritoneal macrophages from each group was determined by flow cytometry. Phagocytosis of Raw264.7 cells from each group was determined by (B) flow cytometry and (C) confocal microscope. Scale bars, 30 µm. Data are shown as mean ± SEM (n = 3). *P < 0.05.