Figure 6.

BSNP-induced trained immunity protects mice against secondary infection. (A) Schematic of in vivo phagocytic test experimental setup (n = 3). (B-C) Macrophages (CD11b+, F4/80+ subset) in peritoneal cells from each group were determined by flow cytometry. (D) Schematic of double-infection experimental setup (n = 5). (E) Blood (upper) and peritoneal lavage fluid (below) from mice 24 h after secondary infection was plated for 16 h. Representative plate shows bacterial colonies. Representative H&E staining of (H) lung sections or (I) liver sections. Scale bars, 100 µm. Histological injury of (F) lungs and (G) livers in different groups scored as described in Materials and Methods. Data are shown as mean ± SEM (n = 5). *P < 0.05; **P < 0.01; ***P < 0.001.