Figure 5.

(A) The mechanism of cancer cell killing using ferumoxytol through altering the polarization of tumor associated macrophages. (B) Quantification of hydrogen peroxide (left) and hydroxyl radicals (right), which indicating their heightened levels in macrophage, cancer cell and ferumoxytol co-cultures. (C) Various concentrations of ferumoxytol suppressed tumor growth when compared with untreated controls. (D) Tumor growth was significantly inhibited by two types of iron formulations (ferumoxytol and ferumoxytran-10) while dextran alone did not. (E) To exclude cross-talk of two tumors in the same mouse, tumor growth was compared for cancer cells that were inoculated in the mammary fat pad unilaterally and bilaterally, with no significant difference in tumor volume being observed. (F) Analysis of MRI of cancer inoculation sites treated and untreated with ferumoxytol. Paraspinal muscle data is included as internal control. (G) Corresponding T₂^*-weighted MRI of a mouse inoculated with cancer cells with (right, red arrow) or without (left, black arrow) ferumoxytol. Reproduced with permission from references 140, 173.