Table 1.
CyTOF antibody panel
| Label | Target | Clone | Providers |
|---|---|---|---|
| 89Y | CD45 | HI30 | Fluidigm |
| 141Pr | CD196 (CCR6) | 11A9 | Fluidigm |
| 142Nd | CD19 | HIB19 | Fluidigm |
| 143Nd | CD45RA | HI100 | Fluidigm |
| 144Nd | CD38 | HIT2 | Fluidigm |
| 145Nd | CD4 | RPA-T4 | Fluidigm |
| 146Nd | CD8a | RPA-T8 | Fluidigm |
| 147Sm | CD195 (CCR5) | REA245 (IgG1) | Miltenyi |
| 148Nd | CD197 (CCR7) | REA546 | Fluidigm |
| 149Sm | CD25 (IL-2R) | 2A3 | Fluidigm |
| 150Nd | CD272 (BTLA)a | Polyclonal (IgG) | RnD system |
| 151Eu | CD278/ICOS | C398.4A | Fluidigm |
| 152Sm | CD95/Fas | DX2 | Fluidigm |
| 153Er | Tim-3 | F38- 2 × 102 | Fluidigm |
| 154Sm | IL1R1a | Polyclonal (IgG) | RnD system |
| 155Gd | CD279 (PD-1) | EH12.2H7 | Fluidigm |
| 156Gd | CD183 (CXCR3) | G025H7 | Fluidigm |
| 158Gd | CD134 (OX40) | ACT35 | Fluidigm |
| 159Tb | FoxP3 | 259D/C7 | Fluidigm |
| 160Gd | CD28 | CD28.2 | Fluidigm |
| 161Dy | IL6Ralphaa | REA291 | Miltenyi |
| 162Dy | CD27 | L128 | Fluidigm |
| 163Dy | CD57 | HCD57 | Fluidigm |
| 164Dy | CD45RO | UCHL1 | Fluidigm |
| 165Ho | CD127 (IL-7Ra) | A019D5 | Fluidigm |
| 166Er | IL1R2a | 34141 | RnDsystems |
| 167Er | SH2D1aa | 782702 | RnDsystems |
| 168Er | CD154 (CD40L) | 24-31 | Fluidigm |
| 170Er | CD152 (CTLA-4) | 14D3 | Fluidigm |
| 171Yb | CD185 (CXCR5) | RF8B2 | Fluidigm |
| 172Yb | Ki-67 | B56 | Fluidigm |
| 173Yb | HLA-DR | L243 | Fluidigm |
| 174Yb | CD56 (NCAM)a | REA196 (IgG1) | Miltenyi |
| 175Lu | CD184 (CXCR4) | 12G5 | Fluidigm |
| 176Yb | CD62L (L-selectin)a | REA615 | Miltenyi |
| 209Bi | CD11b (Mac-1) | ICRF44 | Fluidigm |
a
Manually coupled using the Maxpar X8 Antibody Labeling Kit (Fluidigm).