Figure 6.

Positional peptide library immunogens induce expansion of diverse epitope‐specific CD8⁺ T cell repertoires. Mice were vaccinated with CPQ/A5 or CPQ/Pos1‐3‐5‐8 on days 0 and 7, and tet⁺CD8⁺ T cells were sorted from splenocytes on day 14, followed by DNA extraction and TCR sequencing. A) The 30 most abundant amino acid clonotype frequencies from each sample are multicolored while the remaining cumulative clone frequency is shown in purple. The total number of unique CDR3 clonotypes observed in each sample is noted. B) The top 5 most significantly differentially abundant tet⁺CD8⁺ TCR clones enriched in CPQ/A5 relative to CPQ/Pos‐1‐3‐5‐8 (top) and conversely enriched in CPQ/Pos1‐3‐5‐8 relative to CPQ/A5 (bottom). CDR3 sequences are indicated. C) Repertoire metrics, showing the number of total TCR transcripts detected in each sample, number of unique CDR3 sequences observed, Gini coefficient, clonality, richness of motif and Shannon entropy of the TCR of tet⁺CD8⁺ T cells from CPQ/A5 and CPQ/Pos1‐3‐5‐8 vaccinated mice. The experiment was performed with n = 3 independent mice per group and the line shows the mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, analyzed by unpaired student t test.