FIG. 1.

(A) Expression of WT HA-BAD in IL-3-starved parental and BCR/ABL-expressing 32Dcl3 cells. Extracts from cells starved for 0, 4, and 6 h (32D) or 0, 6, and 12 h (BCR/ABL) were blotted with anti-HA antibody (α-HA). (B) Effect of z-VAD fmk (50 μM) on levels of WT HA-BAD in IL-3-starved parental 32Dcl3 cells. Western blot analyses were performed with an anti-BAD (C-20, upper gels) antibody. Caspase-3 levels were measured as a control for the inhibitory capacity of z-VAD fmk. Results are representative of three separate experiments. (C) HA-BAD expression in subcellular fractions from retrovirus-infected parental cells. At 0, 8, and 12 h after IL-3 withdrawal, mitochondrial (HM) and cytoplasmic (C) extracts were tested for BAD expression using the anti-BAD (C-20) antibody. Arrows indicate full-length HA-BAD; arrows plus asterisks indicate cleaved products of BAD or caspase 3. Results are representative of three separate experiments.