FIG. 2.

Snu17p is a yeast U2 snRNP protein. DNA coding for a tandem repeat of S. aureus protein A was fused 3′ to SNU17. Extracts (50 μl) from the resulting strain (Snu17p-tag), as well as from the parental wild-type strain (No tag), were immunoprecipitated using IgG-agarose and then washed at various NaCl concentrations, as indicated above the lanes. The snRNA content of the precipitates was assayed by Northern blot analysis (snRNAs are indicated). Total refers to the total RNAs from 4 μl of extract (8% of the total input) before immunoprecipitation.