Figure 3.
Activation of Neuregulin signalling pathway demonstrated by immunocytochemistry. (A) Co-immunostaining of Phalloidin and erbB2-℗. To visualise the erbB2-℗ clusters on the cell membrane, two regions of the low magnification image were enlarged. Image a’ and b’ are the higher magnifications of regions a and b respectively. Abundant erbB2-℗ signals (indicated by arrows) were observed only on multi-nuclei bag fibres (b and b’), and rarely observed on the others (a and a′). (B) Immunostaining of Egr3 co-stained with S46. Egr3-positivity was only observed in S46-positive myofibres, confirming its specificity. (C) A bag fibre under higher magnification showing Egr3 staining.
Source: Figure from Guo 59 with permission from Elsevier.