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. 2021 Dec 20;219(3):e20211924. doi: 10.1084/jem.20211924

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© 2021 Atkins et al.

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Figure S4. — Characterization of the embryonic T cell lineage. Related to Fig. 4. (A) Representative flow cytometric analysis of CD3 expression between days 25 and 40 of T lymphoid differentiation in cultures derived from day 6 CD34⁺CD43⁻ or cord blood CD34⁺ cells. SSC-A, side scatter area. (B) Quantification of the frequency of CD3⁺ cells between days 25 and 42 of T lymphoid differentiation in cultures derived from day 6 CD34⁺CD43⁻ (gray) or cord blood CD34⁺ (black) cells (n = 3). ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001; and ****, P < 0.0001 versus day 25 of T lymphoid differentiation for the indicated population and ^#, P < 0.05 versus the stage-matched sample. (C) Representative flow cytometric analysis of TCRγδ and TCRαβ expression between days 25 and 40 of T lymphoid differentiation in cultures derived from day 6 CD34⁺CD43⁻ or cord blood CD34⁺ cells. (D) Representative flow cytometric analysis of Vγ9 and Vδ2 expression between days 25 and 40 of T lymphoid differentiation in cultures derived from day 6 CD34⁺CD43⁻ cells or cord blood CD34⁺ cells. (E) Representative flow cytometric analysis of Vδ1 expression between days 25 and 40 of T lymphoid differentiation in cultures derived from day 6 CD34⁺CD43⁻ or cord blood CD34⁺ cells. (F) Quantification of frequency CD5⁺ cells in the CD34⁺CD7⁺ and CD34⁻CD7⁺ populations derived from day 6 CD34⁺CD43⁻ (gray) or cord blood CD34⁺ (black) cells after 12 d of culture with OP9-DL4 cells (n = 4). ANOVA. **, P < 0.01; ****, P < 0.0001 versus the indicated sample.