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| LB/agar plate | Final concentration | Amount |
|---|---|---|
| Bacto Tryptone | 1 % (w/v) | 10 g |
| Bacto Yeast Extract | 0.5 % (w/v) | 5 g |
| NaCl | 1 % (w/v) | 10 g |
| Agar | 1.5 % (w/v) | 15 g |
| Milli-Q water | n/a | Up to 1000 mL |
| Total | n/a | 1000 mL |
Sterilize by autoclaving at 120°C for 20 min.
After adding filter-sterilized ampicillin (final concentration = 50 μg/mL), pour the medium to polystyrene dishes (diameter 10 cm). Use after the agar hardened.
The plate can be stored at 4°C for up to one month.
