Buffer AW	Final concentration	Amount
1 M Tris-HCl (pH 8.0)	50 mM	50 mL
Glycerol	10 % (v/v)	100 mL
Imidazole	20 mM	1.36 g
Milli-Q water	n/a	Up to 1000 mL
Total	n/a	1000 mL

The medium can be stored at 4°C for up to six months.