Figure 3.

Effect of the number of PG groups in oligonucleotides on their accumulation in A549 cells

(A) Under carrier-free conditions. (B) In the presence of cationic liposomes 2X3-DOPE. A549 cells were incubated with oligonucleotides D8-D14 alone at 5 μM or complexed with liposomes 2X3-DOPE at 1 μM for 4 h. The percentage of fluorescent cells was measured by flow cytometry 4 h post-transfection. Data are presented as mean ± SEM. The data were statistically processed using Student's t test (two-tailed, unpaired); a p value of ≤0.05 was considered to indicate a significant difference; ∗∗data were statistically insignificant. All experimental points were run in triplicate for statistical analysis. C1, control, cells incubated without oligonucleotides; C2, control, cells incubated without liposomes; C(L), cells incubated in the presence of liposomes 2 × 3/DOPE.