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. 2021 Nov 29;27:211–226. doi: 10.1016/j.omtn.2021.11.025

Figure 4.

Figure 4

RNase H cleavage of the MDR1 RNA complexed with ASOs

(A) Autoradiograph of a 12% polyacrylamide/8 M urea gel. Lanes Im and T1, imidazole ladder and partial RNA digestion with RNase T1, respectively; C, control, RNA incubated in the absence of ASOs and RNase H. Lanes 1–5 (left panel), lanes 6 and 7 (right panel), RNA/ASO incubated with RNase H: 1, RNA/M2; 2, RNA/M1; 3, RNA/M3; 4, RNA/G1; 5, RNA/G2; 6, RNA/G3; 7, RNA/G4. MDR1 RNA (0.05 μg) was pre-complexed with 10 μM ASO at 37°C for 1 h and incubated with RNase H (1 U/mL) at 37°C for 30 min. (B) Secondary structure of the 678 nt fragment of MDR1 RNA. The ASO binding site is indicated in blue. Spontaneous cleavages at CA and UA sites of MDR1 RNA are indicated by red arrows.