Table 2.
Polymorphic statistics calculated for three combinations of selective primers for Sporothrix spp.
|
#3 EcoRI-GA/MseI-TT | ||||||||
|
Species (n) |
Fragments |
H |
PIC |
E |
Havp |
MI |
D |
Rp |
| S. brasiliensis (72) | 100 | 0.3781 | 0.3066 | 25.3194 | 0.0001 | 0.0013 | 0.9359 | 21.0278 |
| S. schenckii (67) | 115 | 0.3008 | 0.2555 | 21.2089 | 0.0000 | 0.0008 | 0.9660 | 29.2537 |
| S. globosa (34) | 71 | 0.4478 | 0.3475 | 24.0294 | 0.0002 | 0.0045 | 0.8855 | 14.7647 |
| Clinical clade (173) | 149 | 0.2662 | 0.2308 | 23.5606 | 0.0000 | 0.0002 | 0.9750 | 38.9132 |
| S. mexicana (4) | 36 | 0.4996 | 0.3748 | 18.5000 | 0.0035 | 0.0642 | 0.7377 | 28.0000 |
| S. pallida (3) | 49 | 0.4998 | 0.3749 | 25.0000 | 0.0034 | 0.0850 | 0.7414 | 29.3333 |
| S. chilensis (2) | 31 | 0.4370 | 0.3415 | 21.0000 | 0.0070 | 0.1480 | 0.5447 | 20.0000 |
| S. brunneoviolacea (2) | 36 | 0.4753 | 0.3623 | 22.0000 | 0.0066 | 0.1452 | 0.6299 | 28.0000 |
| S. dimorphospora (2) | 30 | 0.4061 | 0.3236 | 21.5000 | 0.0068 | 0.1455 | 0.4898 | 17.0000 |
| Overall (186) |
153 |
0.2588 |
0.2253 |
23.3709 |
0.000009 |
0.0002 |
0.9766 |
39.9032 |
|
#5 EcoRI-GA/MseI-AG | ||||||||
|
Species (n) |
Fragments |
H |
PIC |
E |
Havp |
MI |
D |
Rp |
| S. brasiliensis (72) | 84 | 0.4100 | 0.3259 | 24.1806 | 0.0001 | 0.0016 | 0.9172 | 20.1389 |
| S. schenckii (67) | 110 | 0.3323 | 0.2771 | 23.1492 | 0.0000 | 0.0010 | 0.9557 | 27.5820 |
| S. globosa (34) | 67 | 0.4438 | 0.3453 | 22.2647 | 0.0002 | 0.0043 | 0.8897 | 15.7059 |
| Clinical clade (173) | 143 | 0.2742 | 0.2366 | 23.4566 | 0.0000 | 0.0002 | 0.9730 | 38.832 |
| S. mexicana (4) | 39 | 0.4815 | 0.3656 | 23.2500 | 0.0031 | 0.0718 | 0.6462 | 31.5000 |
| S. pallida (3) | 50 | 0.4992 | 0.3746 | 24.0000 | 0.0033 | 0.0799 | 0.7713 | 31.3333 |
| S. chilensis (2) | 34 | 0.1107 | 0.1046 | 32.0000 | 0.0016 | 0.0521 | 0.1150 | 4.0000 |
| S. brunneoviolacea (2) | 54 | 0.4957 | 0.3728 | 29.5000 | 0.0046 | 0.1354 | 0.7039 | 49.0000 |
| S. dimorphospora (2) | 30 | 0.4550 | 0.3515 | 19.5000 | 0.0076 | 0.1479 | 0.5814 | 21.0000 |
| Overall (186) |
160 |
0.2511 |
0.2195 |
23.5591 |
0.000008 |
0.0001 |
0.9783 |
40.2795 |
|
#6 EcoRI-TA/MseI-AA | ||||||||
|
Species (n) |
Fragments |
H |
PIC |
E |
Havp |
MI |
D |
Rp |
| S. brasiliensis (72) | 75 | 0.3103 | 0.2622 | 14.4028 | 0.0001 | 0.0008 | 0.9632 | 14.5278 |
| S. schenckii (67) | 100 | 0.2903 | 0.2482 | 17.6268 | 0.0000 | 0.0007 | 0.9689 | 22.0895 |
| S. globosa (34) | 43 | 0.3912 | 0.3147 | 11.4706 | 0.0003 | 0.0031 | 0.9290 | 8.9412 |
| Clinical clade (173) | 126 | 0.2108 | 0.1886 | 15.0924 | 0.0000 | 0.0001 | 0.9856 | 25.3872 |
| S. mexicana (4) | 20 | 0.4688 | 0.3589 | 12.5000 | 0.0059 | 0.0732 | 0.6123 | 11.0000 |
| S. pallida (3) | 28 | 0.4955 | 0.3727 | 15.3333 | 0.0059 | 0.0904 | 0.7031 | 14.0000 |
| S. chilensis (2) | 14 | 0.0000 | 0.0000 | 14.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 |
| S. brunneoviolacea (2) | 42 | 0.4898 | 0.3698 | 24.0000 | 0.0058 | 0.1399 | 0.6764 | 36.0000 |
| S. dimorphospora (2) | 33 | 0.4224 | 0.3332 | 23.0000 | 0.0064 | 0.1472 | 0.5175 | 20.0000 |
| Overall (186) | 138 | 0.1957 | 0.1765 | 15.1720 | 0.000007 | 0.0001 | 0.9879 | 26.1075 |
D: discriminating power; E: effective multiplex ratio; H: expected heterozygosity; Havp: mean heterozygosity; MI: marker index; PIC: polymorphism information content; Rp: resolving power. The clinical clade included S. brasiliensis (n = 72), S. schenckii (n = 67), and S. globosa (n = 34). S. luriei (n = 1) and S. stenoceras (n = 1) were not included in the analysis. A comprehensive view polymorphic statistics is presented in Supplementary Table S4.