Supplemental Figure 2.

Microbiota-based vaccination with a specific dose of extracts from different gut segments improves blood glucose control in male mice. A) Vaccination model of control diet (CD)-fed male mice given a single subcutaneous injection with extracts prepared from different intestinal segments (upper small intestine (SI), cecum, colon) or from a homogenized fecal pellet (‘fecal’) from male mice. Glucose tolerance was assessed 5 weeks after the vaccination event. B) Body mass. Blood glucose vs. time and quantified area under the curve during a glucose tolerance test (GTT) (2 g/kg, i.p.) in CD-fed WT male mice 5 weeks after vaccination (5000x dilution of upper SI, colon and fecal extracts and a range of 500–20,000x dilutions of cecal extracts were tested; n = 9–10). ∗Denotes significant difference from control group injected with saline (pbs) determined by one-way ANOVA (p < 0.05). Each symbol represents a mouse and other values shown are mean +/− SEM.