Figure 5.

A prime-boost vaccination strategy is required to improve glucose control in obese male mice. A) Vaccination model during diet-induced obesity where mice were fed a HFD for 8 weeks before the first lower SI extract injection and glucose tolerance was assessed 4 weeks after the initial injection. A second ‘boost’ injection of lower SI extract was delivered, and glucose tolerance was assessed 4 weeks after the second injection, which equated to a total of 16 weeks of HFD feeding. B) Body mass, blood glucose vs. time and quantified area under the curve during a glucose tolerance test (GTT) (1 g/kg, i.p.) in 12wk HFD-fed WT male mice vaccinated a single time, according to Fig 5A (n = 8). C) Body mass, blood glucose vs. time and quantified area under the curve during a GTT (1 g/kg, i.p.) in 16wk HFD-fed WT male mice vaccinated twice, according to Fig 5A (n = 8). D) Experimental vaccination model of mice given a “prime” injection of lower SI extract during control diet (CD)-feeding in mice. Five weeks after the prime injection, mice were switched to a HFD for 8 weeks and then a second “boost” injection of lower SI extract was delivered. Four weeks later, after 12 total weeks of HFD feeding, glucose tolerance was assessed. E) Body mass, blood glucose vs. time and quantified area under the curve during a GTT (1 g/kg, i.p.) in 12wk HFD-fed WT male mice vaccinated twice according to Fig 5D (n = 9–10). ∗Denotes significant difference from control group injected with saline (pbs) determined by t-test (p < 0.05). Each symbol represents a mouse and other values shown are the mean +/− SEM.