Fig. 5.

Illustration of mutated amino acid positions in different Lcn2-based random libraries using similar graphical visualization as in Fig. 3D. (A) First-generation library designed to select Anticalins specific for protein targets (Schönfeld et al., 2009). (B) Second-generation library designed to select Anticalins specific for small-molecule targets (Kim et al., 2009). (C) Third-generation library designed to select Anticalins against protein and peptide antigens as well as hapten targets building upon structural analyses of Anticalins successfully selected from the first two libraries (Gebauer et al., 2013).