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. 2021 Dec 2;25(1):103558. doi: 10.1016/j.isci.2021.103558

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

LIPUS regulates the G2/M checkpoint and reduces stem cell characteristics of GSC

(A) The BrdU and 7-AAD analysis of GSCs with LIPUS treatment by flow cytometry, n = 3.

(B) Western blotting analysis expression of the key proteins of the G2/M checkpoint. The results represent ratios of each protein and GAPDH levels and are normalized to Ctrl.

(C) Western blotting analysis expression of the key nodes of the Hedgehog (Hh) and Wnt/β-catenin pathways. The results represent ratios of each protein and GAPDH levels and are normalized to Ctrl. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001.