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. 2021 Dec 2;25(1):103558. doi: 10.1016/j.isci.2021.103558

Figure 3.

Figure 3

LIPUS shortens the telomere length and leads to telomere damage

(A) qPCR analysis of the telomere length (T/S) in GSCs with LIPUS treatment. n = 3.

(B) Telomere metaphase spread and telomere fluorescence in situ hybridization analysis in GSCs with LIPUS treatment (red arrow indicates telomeric loss and white arrow indicates fragile telomeres). Quantification of incomplete telomere. Each dot for a metaphase. Medians (bar) from three independent experiments (n = 30 for each experiment).

(C) Telomere fluorescence in situ hybridization of TIFs in GSCs with LIPUS treatment (red for 53 bp1 and green for telomeres). Quantification of the average (av.) number of TIFs per nuclei. n = 3, at least 30 cells per experiment. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001.