REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
BD Pharmingen™ PE mouse anti-human CD133 | BD Pharmingen | Cat #566594 RRID: AB_2744281 |
BD Pharmingen™Alexa Fluor® 647 mouse anti-nestin | BD Pharmingen | Cat #560393 RRID: AB_1645170 |
FITC anti-BrdU flow kit | BD Pharmingen | Cat # 559619 RRID: AB_2617060 |
APC Annexin V | BD Pharmingen | Cat #550475 RRID: AB_2868885 |
Recombinant Anti-CD133 antibody | Abcam | ab216323 RRID: AB_2847920 |
Anti-estin antibody | Abcam | ab18102 RRID: AB_444246 |
Recombinant Anti-Ki67 antibody | Abcam | ab279653 RRID: AB_302459 |
Fluorescein (FITC)–conjugated Affinipure goat anti-mouse IgG(H + L) | Proteintech | SA00003-1 RRID: AB_2890896 |
CoraLite594 – conjugated goat anti-rabbit IgG(H + L) | Proteintech | RRID: AB_2810984 |
53BP1 antibody | Novus Biologicals | NB100-304 |
GLI1 (V812) antibody | Cell Signaling Technology | #2534 RRID: AB_2294745 |
PTCH1 (C53A3) rabbit mAb | Cell Signaling Technology | #2468 RRID: AB_2300741 |
Wnt3a (C64F2) rabbit mAb | Cell Signaling Technology | #2721 RRID: AB_2215411 |
Wnt5a/b (C27 × 108) rabbit mAb | Cell Signaling Technology | #2530 RRID: AB_2215595 |
β-Catenin (D10A8) XP® Rabbit mAb | Cell Signaling Technology | #8480 RRID: AB_11127855 |
PLK1 (208G4) rabbit mAb | Cell Signaling Technology | #4513 RRID: AB_2167409 |
cdc2 (POH1) mouse mAb | Cell Signaling Technology | #9116 RRID: AB_2074795 |
Cyclin B1 (D5C10) XP® Rabbit mAb | Cell Signaling Technology | #12231 RRID: AB_2783553 |
GAPDH (D16H11) XP® Rabbit mAb | Cell Signaling Technology | #5174 RRID: AB_10622025 |
Anti-mouse IgG, HRP-linked Antibody | Cell Signaling Technology | #7076 RRID: AB_330924 |
Anti-rabbit IgG, HRP-linked antibody | Cell Signaling Technology | #7074 RRID: AB_2099233 |
Chemicals, peptides, and recombinant proteins | ||
CCk-8 kit | ZETA | No.: k009 |
TRIzol™ LS reagent | Invitrogen | Lot 264006 |
RT-PCR kit | Abgen biotechnology | 06-104 |
ChamQ Universal SYBR qPCR master mix | Vazyme Biotech | Q711-02 |
RIPA buffer(high) | Solarbio | R0010 |
DNA extraction kit | Biogama | BW-GD2211-01 |
Colchicine solution | Biological Industries | 1952210 |
JC-1 detection kit | Dojindo Molecular Technology | MT09 |
Singlet oxygen detection kit | Bestbio | BB-47055 |
Singlet oxygen detection kit | Invitrogen | S36002 |
7-ADD | BD Pharmingen | Cat # 559925 RRID: AB_2869266 |
TelC-FITC PNA probe | Panagene | F1009 |
Recombinant human yt c | Novoprotein | No.:CF80 |
Recombinant humanCyt c1 | Abnova | Cat#H00001537-p02 |
Recombinant human CYB5A(N-6His) | Novoprotein | #C217 |
Recombinant human CYB5B(C-6His) | Novoprotein | #CJ36 |
N-2 Supplement (100X) | Gibco | 2245840 |
B-27 Supplement (50X) | Gibco | 2234290 |
Recombinant human EGF | PerproTech | AF-100-15 |
Recombinant human FGF basic | PerproTech | 100-18B |
Temozolomide | Sigma | T2577 |
Mito-TEMPO | GLPBIO | GC31682 |
Colorimetric TUNEL apoptosis assay kit | Beyotime | C1098 |
MEM (1X) | Gibco | 11095080 |
DMEM basic (1X) | Gibco | C11995500BT |
DMEM/F-12 (1:1) basic (1X) | Gibco | C11330500BT |
Experimental models: cell lines | ||
U87MG | BNCC | BNCC100646 |
A172 | iCell Bioscience Inc | N/A |
TBD0220 | Tianjin Medical University General Hospital | N/A |
Experimental models: organisms/strains | ||
Mouse: BALB-c nude mice | the Experimental Animal Center of Academy of Military Medical Sciences, China | N/A |