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. 2021 Dec 2;25(1):103558. doi: 10.1016/j.isci.2021.103558
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

BD Pharmingen™ PE mouse anti-human CD133 BD Pharmingen Cat #566594 RRID: AB_2744281
BD Pharmingen™Alexa Fluor® 647 mouse anti-nestin BD Pharmingen Cat #560393 RRID: AB_1645170
FITC anti-BrdU flow kit BD Pharmingen Cat # 559619 RRID: AB_2617060
APC Annexin V BD Pharmingen Cat #550475 RRID: AB_2868885
Recombinant Anti-CD133 antibody Abcam ab216323 RRID: AB_2847920
Anti-estin antibody Abcam ab18102 RRID: AB_444246
Recombinant Anti-Ki67 antibody Abcam ab279653 RRID: AB_302459
Fluorescein (FITC)–conjugated Affinipure goat anti-mouse IgG(H + L) Proteintech SA00003-1 RRID: AB_2890896
CoraLite594 – conjugated goat anti-rabbit IgG(H + L) Proteintech RRID: AB_2810984
53BP1 antibody Novus Biologicals NB100-304
GLI1 (V812) antibody Cell Signaling Technology #2534 RRID: AB_2294745
PTCH1 (C53A3) rabbit mAb Cell Signaling Technology #2468 RRID: AB_2300741
Wnt3a (C64F2) rabbit mAb Cell Signaling Technology #2721 RRID: AB_2215411
Wnt5a/b (C27 × 108) rabbit mAb Cell Signaling Technology #2530 RRID: AB_2215595
β-Catenin (D10A8) XP® Rabbit mAb Cell Signaling Technology #8480 RRID: AB_11127855
PLK1 (208G4) rabbit mAb Cell Signaling Technology #4513 RRID: AB_2167409
cdc2 (POH1) mouse mAb Cell Signaling Technology #9116 RRID: AB_2074795
Cyclin B1 (D5C10) XP® Rabbit mAb Cell Signaling Technology #12231 RRID: AB_2783553
GAPDH (D16H11) XP® Rabbit mAb Cell Signaling Technology #5174 RRID: AB_10622025
Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology #7076 RRID: AB_330924
Anti-rabbit IgG, HRP-linked antibody Cell Signaling Technology #7074 RRID: AB_2099233

Chemicals, peptides, and recombinant proteins

CCk-8 kit ZETA No.: k009
TRIzol™ LS reagent Invitrogen Lot 264006
RT-PCR kit Abgen biotechnology 06-104
ChamQ Universal SYBR qPCR master mix Vazyme Biotech Q711-02
RIPA buffer(high) Solarbio R0010
DNA extraction kit Biogama BW-GD2211-01
Colchicine solution Biological Industries 1952210
JC-1 detection kit Dojindo Molecular Technology MT09
Singlet oxygen detection kit Bestbio BB-47055
Singlet oxygen detection kit Invitrogen S36002
7-ADD BD Pharmingen Cat # 559925
RRID: AB_2869266
TelC-FITC PNA probe Panagene F1009
Recombinant human yt c Novoprotein No.:CF80
Recombinant humanCyt c1 Abnova Cat#H00001537-p02
Recombinant human CYB5A(N-6His) Novoprotein #C217
Recombinant human CYB5B(C-6His) Novoprotein #CJ36
N-2 Supplement (100X) Gibco 2245840
B-27 Supplement (50X) Gibco 2234290
Recombinant human EGF PerproTech AF-100-15
Recombinant human FGF basic PerproTech 100-18B
Temozolomide Sigma T2577
Mito-TEMPO GLPBIO GC31682
Colorimetric TUNEL apoptosis assay kit Beyotime C1098
MEM (1X) Gibco 11095080
DMEM basic (1X) Gibco C11995500BT
DMEM/F-12 (1:1) basic (1X) Gibco C11330500BT

Experimental models: cell lines

U87MG BNCC BNCC100646
A172 iCell Bioscience Inc N/A
TBD0220 Tianjin Medical University General Hospital N/A

Experimental models: organisms/strains

Mouse: BALB-c nude mice the Experimental Animal Center of Academy of Military Medical Sciences, China N/A