Fig. 1.

Correlation and association of CCND1 DNA methylation with mRNA expression, CCND1 amplification, HPV status, and sensitivity to FGFR-targeted TKIs. a Genomic context of 30 selected CpG sites within CCND1 included into the present study. The illustration, including the predicted regulatory build [63] and transcription start sites according to the Eponine algorithm [64], was exported from www.ensemble.org [65]. b Methylation levels of the 30 selected CpG loci within CCND1 and mRNA expression levels in NATs and tumor tissues, HPV(−) and HPV(+) tumor tissues, and CCND1 amplified and non-amplified tumors. c Spearman’s ρ of correlations between CCND1 DNA methylation and CCND1 mRNA expression. d Spearman’s ρ of correlations between CCND1 DNA methylation and mRNA expression with CCND1 amplification. e Spearman’s ρ of correlations of CCND1 DNA methylation, mRNA expression, and amplification with ln(IC₅₀) of PD 173074 and AZD4547. Statistically significant features are marked with asterisks*. P values (corrected and uncorrected) refer to Wilcoxon-Mann–Whitney U test for comparisons and to Spearman’s ρ for correlations analysis, respectively