Fig. 2.

Correlation and association of FGFR1 DNA methylation with mRNA expression, FGFR1 amplification, HPV status, and sensitivity to FGFR-targeted TKIs. a Genomic context of thirteen selected CpG sites within FGFR1 included into the present study. The illustration, including the predicted regulatory build [63] and transcription start sites according to the Eponine algorithm [64], was exported from www.ensemble.org [65]. b Methylation levels within the thirteen selected CpG loci within FGFR1 and mRNA expression levels in NAT and tumor tissues, HPV(−) and HPV(+) tumors, and FGFR1 amplified and non-amplified tumors. c Spearman’s ρ of correlations between DNA methylation and FGFR1 mRNA expression. d Spearman’s ρ of correlations between FGFR1 DNA methylation and mRNA expression with FGFR1 amplification. e Spearman’s ρ of correlations of FGFR1 DNA methylation, mRNA expression, and amplification with ln(IC₅₀) of PD 173074 and AZD4547. Statistically significant features are marked with asterisks *. P values (corrected and uncorrected) refer to Wilcoxon-Mann–Whitney U test for comparisons and to Spearman’s ρ for correlations analysis, respectively