Fig. 5. Effects of CA and its amino acid ester-coupled analogues 6c–f (10 μmol L−1) on the mRNA levels of lipogenic genes SREBP1c, ACC and FAS in OA-elicited HepG2 cells. Real-time quantitative PCR was conducted with gene-specific oligonucleotide primers. The amplification of β-actin served as an internal control (n = 6). Data are depicted as means ± SD in triplicate. **p < 0.01, ***p < 0.001, test groups vs. negative control group. Ctrl: negative control; CA: chlorogenic acid; SREBP1c: sterol response element binding protein 1c; ACC: acetyl-CoA carboxylase; FAS: fatty acid synthase.
