Figure 3.

IL-18R blockade preserves ST-HSCs and mitigates quiescence

WT (C57BL/6) mice were inoculated with 10⁵ copies of IOE and BM was analyzed at 7 dpi.

(A) Model depicting treatment of WT mice with either IL-18Rα blocking antibody or an isotype control.

(B–E) Absolute cellularity of (B) BM, (C) HSPCs, (D) LT-HSCs, and (E) ST-HSCs at 7 dpi, n = 3–8 mice/group.

(F and G) The frequency of quiescent (F) LT-HSCs and (G) ST-HSCs, n = 3–8 mice/group.

(H) Bacterial burden in the spleen of IOE-infected mice treated with either isotype or anti-IL-18Rα, quantified by copies of the dsb gene.

(I and J) Cell death breakdown of (I) LT-HSC and (J) ST-HSCs stained with 7AAD and Annexin V in mock- or IOE-infected WT mice treated with mock, isotype control, or anti-IL18Rα, n = 7–9 mice/group. ^∗p < 0.05, ^∗∗p < 0.001, ^∗∗∗p < 0.0001.