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. 2021 Nov 24;16(12):3064–3075. doi: 10.1016/j.stemcr.2021.10.017

Figure 3.

Figure 3

Genetic safety and robustness of base editing and simultaneous hiPSC generation

(A) PCR to detect episomal vector retention into four hiPSC lines reprogrammed in absence of the ABEmax construct (−ABE) and eight independently base-edited hiPSC lines (+ABE). Two different plasmid regions are targeted in these PCRs: EBNA-1 and OriP. Water control (−), positive control (+), NOTCH3: patient 1 (Pt 1) and patient 2 (Pt 2). LDLR: patient 3 (Pt 3) and patient 4 (Pt 4).

(B) Karyotyping of a representative base-edited hiPSC lines and its non-ABE control.

(C) Bar graphs depicting the A-to-G editing frequency on the targeted loci: gRNA2 (chr11:-5254881) and Site 16 (chr1:-179826685). Each point represents an independent event of simultaneous reprogramming and base editing on primary fibroblasts derived from five different donors (n = 5). Data are represented as mean + standard deviation.