Figure 1.

Teratoma-derived skeletal myogenic progenitors are expandable in vitro

(A) Schematic of in vitro expansion of teratoma-derived skeletal myogenic progenitors. Four-week EGFP-labeled mouse ESC-derived teratomas were harvested and FACS sorted for the skeletal myogenic population: CD31⁻ CD45⁻ α7-integrin⁺ VCAM-1⁺ (α7⁺ V⁺) cells. The freshly sorted α7⁺ V⁺ cells (“Fresh”) were subsequently cultured and expanded for another 37 days over 8 passages (“Expanded”).

(B) In vitro cultures of α7⁺ V⁺ cells showed exponential growth for up to 37 days. Data are shown as the mean ± SEM of three independent experiments.

(C) FACS profiling of total teratoma cells, freshly sorted α7⁺ V⁺ cells, and expanded 37-day cells (representative of three independent experiments).

(D) Quantitation of α7⁺ V⁺ and α7⁺ V⁻ cells by FACS during in vitro expansion. Data are shown as the mean ± SEM of three independent experiments.

ESCs, embryonic stem cells; α7, α7-integrin; V, VCAM-1.

See also Figure S1.