Figure 2.

In vitro characterization of expanded teratoma-derived skeletal myogenic progenitors

(A) Immunoblots (top) and quantification (bottom) showing that both freshly sorted and expanded cells expressed the muscle stem cell transcription factor PAX7 (two independent samples from each group are shown). Data are shown as the mean ± SEM of five independent experiments.

(B) Immunostaining (left) and quantification (right) showing the expression of PAX7 and MYOD1 in 3-day cultures of freshly sorted and expanded cells. Scale bar represents 50 μm. Data are shown as the mean ± SEM of three independent experiments.

(C) Immunostaining (top) of MHC in freshly sorted and expanded cells cultured in differentiation medium. Scale bar represents 200 μm. Quantification of differentiation (bottom left) and fusion in MHC⁺ myotubes (bottom right) from three independent experiments. Data are shown as the mean ± SEM.

(D) Clonal analysis (top) showing that both single freshly sorted and expanded cells were capable of forming MHC⁺ skeletal myogenic colonies. Scale bar represents 200 μm. Quantification of clonal efficiency (bottom left) and clonal size distribution (bottom right) from three independent experiments and 170 single cells per experiment. ^∗∗p < 0.01. Data are shown as the mean ± SEM.

α7, α7-integrin; V, VCAM-1; MHC, myosin heavy chain.

See also Figure S2.