Figure 3.

Bulk RNA sequencing analysis of differential expressed genes and pathways during CM maturation

(A and B) Gene ontology pathway enrichment analysis identified (A) upregulation and (B) downregulation of key pathways associated with matured CMs (low GFP) compared with fetal CMs (high GFP).

(C) Correlated clustered heatmap of a list of top 24 transcription factors in the top 8 up-/downregulated pathways and matured (low GFP) CMs compared with fetal CMs (high GFP). The color intensity represents column Z score, with red indicating high expression and green indicating low expression. For (A)–(C), n = 3 independently differentiated groups, p < 0.05, FDR < 0.1.

(D) CNet plot identified the interaction of genes involved in the interferon-signaling pathway. The color intensity represents fold-change.