Figure 6.

Electrophysiological assessment of calcium transients in IFN-γ and ruxolitinib-treated H7-GCaMP6s-derived CMs

(A) Representative electrophysiological image of H7-GCaMP6s expressing CMs recorded 3, 7, and 10 days post IFN-γ treatment.

(B)–(E) Representative data of one of the two H7-GCaMP6s clone-derived CMs that were differentiated and analyzed. Dot plot showing mean (B) calcium transient duration (s), (C) calcium transient peak intensity (Au), (D) depolarization speed (Au/s), and (E) repolarization duration (30th, 60th, and 90th percentiles) per cell, measured across 30 s, n = 50 cells, three independently differentiated groups, two clones per cell line (H7). Results obtained using the ES03 cell line are presented in Figure S6. Data information: statistical analysis was performed using Student’s two-tailed t test. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.