Fig. 4.

PSA expression and cell cycle analysis of the D3 clone in response to ralaniten and enzalutamide. a Western blot analysis of cell lysates prepared from D3 or parental LNCaP95 cells incubated with androgen (R1881, 1 nM) for 48 hours, n=2. b Cell cycle analysis of D3 cells incubated in the absence of androgen with vehicle (DMSO), enzalutamide (ENZ), EPI-002, EPI-7170 for 48 hours before labeling with BrdU for two hours. The blue arrow indicates a proportion of cells with DNA content >4n and the red arrow shows BrdU-negative cells induced by EPI-7170. *BrdU-negative subpopulation in S phase. c Stacked bar graph summarizes the average cell cycle distribution from n=3 independent experiments