Figure 2:

Effects of the S3 flavonoid on the activities of different hyaluronidases. (A) Effects of 20 μM S3 (“+”) on HA digestion by BTH, recombinant Hyal1, recombinant PH20 (rPH20), and Streptomyces hyaluronidase compared to enzymes treated with vehicle alone (“-“). The “HA” lane was incubated without S3 but with the vehicle from the hyaluronidases. (B) Effects of the S3 flavonoid on HA digestion by lysates from HEK-293 cells transfected with empty vector (“vector”) or cells transfected with Hyal1, Hyal2, or PH20 compared to HA digested by rPH20 or BTH or to undigested HA “HA”. (C) Effects of different concentrations of S3 or vehicle alone (DMSO - “D”) on HA digestion by HEK-293 cells either untransfected (“untrans”), transfected with vector alone, or transfected with an expression vector carrying the cDNA for Cemip. (D) Quantification of the inhibition of HA digestion by S3 (20 μM) in the presence of recombinant Hyal1, recombinant PH20, BTH, or Streptomyces hyaluronidase. *p<0.01. (E) Quantification of HA digestion in live cell cultures of HEK-293 cells treated with S3 (20 μM) following transfection with either Hyal1, Hyal2, PH20 or Cemip expression vectors. *p<0.005.