Figure 6.

Robotic scalable production of hiPSC (LiPSC-GR1.1)-derived human neurons

(A) Neuronal differentiation strategy established for automated cell culture.

(B) Phase-contrast image showing a typical neuronal culture (day 30). Scale bar, 200 μm.

(C) Neurons develop a dense network of neurites upon maturation (day 50). Scale bar, 200 μm.

(D–F) hiPSC-derived cortical neurons (day 40) immunostained for (D) TUJ1 and CUX1, (E) MAP2 and CTIP2, and (F) vGLUT. Scale bar, 50 μm.

(G) hiPSC-derived neuronal cells (day 40) showing immunoreactivity for inhibitory neurotransmitter GABA. Scale bar, 20 μm.

(H) Robotic MEA platform used for high-throughput electrophysiology and functional cell characterization.

(I and J) Comparison of (I) spontaneous neuronal spikes and (J) spike amplitudes in hiPSC-derived cultures after 6 weeks of manual or robotic cell differentiation as measured by MEA. Representative data are expressed as mean ± SD, n > 3 biological replicates (B–G, I, J).

p > 0.5, unpaired t test.