FIG. 5.

Recycling of FM4-64 and GFP-Snc1p in SCF-deficient cells. (A) Wild-type (K699), cdc4-1 (YMT668), cdc34-2 (YMT670), cdc53-1 (YMT740), skp1-12 (Y554), and rbx1-1 (rbx1-1) cells were analyzed for their ability to recycle FM4-64 as described in Materials and Methods. (B) cdc4-1 (YMT668), cdc34-2 (YMT670), skp1-12 (Y554), cdc53-1 (YMT730), and rbx1-1 (rbx1-1) cells transformed with the plasmid JMG118 (GFP-Snc1p) were grown in selective medium at 25°C to early log phase, shifted for 3 h to 37°C, and examined for both localization (left panel) and phosphorylation (right panel) of GFP-Snc1p as described in the legend to Fig. 3. Note that SCF-deficient cells accumulate with the typical hyperpolarized morphology.