TABLE 3.
Two-hybrid analysis of the interaction between Rcy1p and Skp1pa
| AD | DBD | Miller units (mean ± SD) |
|---|---|---|
| Skp1 | None | 59 ± 11 |
| None | Skp1 | 6 ± 4 |
| Rcy1 | None | 9 ± 5 |
| None | Rcy1 | 3 ± 4 |
| Skp1 | Rcy1 | 1,818 ± 134 |
| Rcy1 | Skp1 | 2,187 ± 156 |
| Rcy1-3A | Skp1 | 631 ± 73 |
| Rcy1-ΔF | Skp1 | 91 ± 12 |
a
Skp1 and Rcy1p were fused to either the LexA DBD or the B42 transcriptional AD. Expression of the β-galactosidase reporter was quantified as described in Materials and Methods. Note that Rcy1p interacts with Skp1p in an F-box-dependent manner.