1 |
|
Na2CO3, sodium ascorbate, H2O, 35 °C, 3 h |
2 |
|
Sodium borate buffer, MDAc/H2O, 20 °C,16 h |
3 |
|
90% aq. DMSO, RT, 1 h |
4 |
|
DMSO/H2O/glycerol (2 : 1 : 0.2), LED array |
5 |
|
MeCN : H2O, DMSO, Et3N, borate buffer, RT, 16 h |
6 |
|
Borate buffer, H2O, RT |
7 |
|
1.6 mM cyclization reagent |
|
20% MeCN |
|
80% NH4HCO3 buffer, pH8 |
|
30 °C, 2 h; TCEP |
|
NH4HCO3 buffer, pH 8, RT, 1 h |
8 |
|
pH 9.5 borate buffer, viologen; 80 °C, 12 h |
9 |
|
pH 8.2 borate, Na2CO3; pH 8.0 phose. Buffer, PyAOP; CH3CN, buffer, heat |
10 |
|
TFA, CH2Cl2, |
11 |
|
i-PrOH/NMP (1 : 1) |
|
pH 5.5 phosphate buffer |
12 |
|
H2O, micellar catalysis |
13 |
|
ACN/H2O, 50 °C |