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Reconstitution of the core autophagy machinery allows Atg1 substrate identification in vitro
(A) Overview of the S. cerevisiae core autophagy machinery and its respective functions. Autophagosome formation is initiated at the PAS, requiring both Atg1 kinase and PP activity.
(B) SDS-PAGE analysis of recombinant Atg proteins expressed and purified for this study. Asterisks, co-purifying insect cell proteins.
(C) Autoradiograph of in vitro kinase assays depicting Atg1 substrates.
