Fig. 3.

Mapping of the 5′ ends of the gbaAB-SACOL2595-97 and SACOL2592-90 operons and the 9-6-9 bp inverted repeat as GbaA operator in S. aureus (A). 5′ RNA-seq was used to map TSS-1 and TSS-2 upstream of the divergent gbaAB and SACOL2592-90 operons, respectively, which is displayed with Read-Explorer. (B) The promoter sequence of the gbaAB operon and the 9-6-9 bp inverted repeat are highly conserved across different Staphylococcus species. The promoter regions were aligned using Clustal Omega and presented in Jalview. Intensity of the blue color gradient is based on 50% nucleotide sequence identity. (C) The conservation of the gbaA −10 promoter region and the GbaA operator is further displayed with WebLogo. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)