Fig. 4.

Northern blot analysis of transcription of the gbaAB-SACOL2595-97 and SACOL2592-90 operons under diamide, AGXX®, NEM and MG stress in the S. aureus COL gbaA mutant and the gbaA, gbaAC55S and gbaAC104S complemented strains. Transcription of the gbaAB-SACOL2595-97 (A,B) and SACOL2592-90 operons (C,D) was analyzed in the S. aureus gbaA deletion mutant and in the gbaA, gbaAC55S, gbaAC104S complemented strains before (co) and 30 min after treatment with 2 mM diamide (Dia), 5 μg/ml AGXX® (AG), 0.3 mM NEM and 2 mM MG using Northern blots. The arrows point toward the transcript sizes of the gbaAB-SACOL2595-97 or SACOL2592-90 operons. The methylene blue bands denote the 16S and 23S rRNAs as RNA loading controls below the Northern blots. Band intensities of the gbaAB and SACOL2592-90 operon transcripts were quantified using ImageJ and the data are shown in Figs. S6A–D.