Fig. 9.

The Cys55 and Cys104 residues of GbaA are required for NEM and MG tolerance. Survival assays were performed for the S. aureus gbaA mutant complemented with gbaA, gbaAC55S and gbaAC104S alleles. Strains were grown in RPMI until an OD₅₀₀ of 0.5 and treated with 0.3 mM NEM (A), 2 mM MG (B) and 0.5 mM allicin (C) to determine CFUs after 2 and 4 h of stress exposure. The percentage survival was normalized to the control. Error bars represent the standard deviation (SD) calculated from three biological replicates. The statistics was determined using a Student's unpaired two-tailed t-test. Symbols are: *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001.