FIGURE 2:

Mrx8 is required for efficient cellular respiration. (A) Shown are 10-fold serial dilutions of MRX8 and Δmrx8 cells on glucose and glycerol plates and incubated at the indicated temperatures. (B) MRX8 and Δmrx8 cells were initially cultured in either glucose (left) or glycerol (right) and then diluted into fresh glycerol media and incubated at 30°C. Optical densities were measured at 600 nm at the indicated time, and each data point is an average value of six independent colonies cultured in parallel. Tables indicate doubling time for each strain. (C) Mitochondria were isolated from wild-type cells cultured in glucose, galactose, and ethanol medium. Equal amounts of mitochondrial protein were separated by SDS–PAGE and subjected to immunoblot analysis. Samples were analyzed using antibodies to Mrx8 and Mtg2.