FIGURE 7:

Mss51 migration on a sucrose density is independent of Mrx8. (A) Mitochondria from MSS51-GST cells carrying the Δmrx8 allele cultured at 16°C were lysed and separated by ultracentrifugation on a 5–30% sucrose gradient containing either (top) 10 mM MgOAc, 100 mM NH₄Cl, or (bottom) 10 mM MgOAc, 500 mM NH₄Cl. Fractions were TCA precipitated, separated by SDS–PAGE, and subjected to immunoblot analysis. Antibodies used were against GST (to detect Mss51), Mrp7(bL27m), and Mrp13(mS44). The migration of the 37S and 54S peaks were labeled based on immunoblot analysis. Shown are 10-fold serial dilutions of (B) Δpet309 cells expressing PET309-3HA(2µ), empty vector, or MRX8(2µ), (C) Δmss51 cells expressing MSS51-GST, empty vector, or MRX8(2µ), (D) Δmrx8 cells expressing MRX8, empty vector, or PET309-3HA(2µ), and (E) Δmrx8 cells expressing MRX8, empty vector, or MSS51-GST on glucose and glycerol media at either 30°C or 16°C.