FIGURE 8:

Human MRX8 (GTPBP8) complements Δmrx8 cells and localizes to mitochondria in mammalian cell lines. (A) Shown are 10-fold serial dilutions of Δmrx8 cells expressing MRX8, empty vector, or MTS-tagged GTPBP8 on glucose and glycerol at 16°C. (B) Shown are representative confocal images of HEK293 cells transfected with either empty vector EGFP-N1 or EGFP-tagged GTPBP8. Scale bars: 10 µm (vector) and 5 µm (GTPBP8-EGFP). (C) Mitochondria were isolated from HEK293 cells transfected with EGFP-tagged GTPBP8 and empty vector EGFP-N1 as described in Materials and Methods. (D) HEK293 cell extracts (CE) were fractionated into cytosol (C) and mitochondria (M). Protein samples were separated by SDS–PAGE and subjected to immunoblot analysis. Antibodies used were against GFP (to detect GTPBP8), GAPDH, and COX IV.