TABLE 1:

Genome engineering of PLEKHA5 and PLEKHA6 in mCCD, Hap1, and MDCK cells.

Cell line	Species	CRISPR target(s)	Cell background	Genotyping primers
mCCD PLEKHA6 KO	Mouse	GGTTCATAGAGCTTTTGCGC	mCCD N64 WT	gaggaattcCCAAGTTACCCCGAGAAGGG
		GCCAGTCTTTTATGACGAGC		gagaagcttGGGAGGAGAGGACGTACCAT
mCCD PLEKHA6/7 KO	Mouse	GGTTCATAGAGCTTTTGCGC	mCCD N64 PLEKHA7 KO ( Guerrera et al., 2016; Shah et al., 2016, 2018)	gaggaattcCCAAGTTACCCCGAGAAGGG
		GCCAGTCTTTTATGACGAGC		gagaagcttGGGAGGAGAGGACGTACCAT
Hap1 PLEKHA5 KO	Human	AATGCACCGGTTGTCAGACG	Hap1 WT	CAGGTAGGACAAAATACTGCCAC
				CTGAAACCTAGCTGCAAACTGG
Hap1 PLEKHA5/7 KO	Human	AATGCACCGGTTGTCAGACG	Hap1 PLEKHA7 KO ( Popov et al., 2015; Shah et al., 2018)	CAGGTAGGACAAAATACTGCCAC
				CTGAAACCTAGCTGCAAACTGG
MDCK PLEKHA5 KO	Dog	TGGACTTACGGGATCACCCG	MDCK II Tet-off WT	N/A
MDCK PLEKHA6 KO	Dog	CCACCCGAATGTTGATGAGC	MDCK II Tet-off WT	gaggaattcAAGCTGCTGGGCAAATTGTGTGA
				gagaagcttCCCAGAACTACCGTCCAAGCAGCC