TABLE 1:
Sample | Mean | Mean expected | SD | SD expected | Sample size | Runtime/trace (s) |
---|---|---|---|---|---|---|
R09 | 6.7 (0.1) | 6.3 | 1.9 (0.1) | 1.4 | 197;13;1 | 25 |
R20 | 14.2 (0.2) | 14 | 6.1 (0.2) | 2.1 | 636;43;2 | 53 |
R35 | 22.6 (0.5) | 24.5 | 8.6 (0.5) | 2.7 | 499;25;2 | 168 |
R09* | 6.0 (0.1) | 6.3 | 2.2 (0.1) | 1.4 | 1667;5;1 | 19 |
Y09 | 7.9 (0.1) | 6.3 | 1.6 (0.1) | 1.4 | 853;12;1 | 88 |
SEs in parentheses as extracted from least-squares fitting. The expected values are calculated assuming a 70% labeling efficiency. *Measurement on alternative microscope setup with sCMOS detector. Sample size: number of traces; measurements; independent experiments.