FIGURE 3:

Fap115 is a ciliary protein. (A) Fap115-mCherry localizes to cilia and colocalizes with the basal body protein centrin (antibody staining). (B) GFP-Fap115 localizes to cilia and basal bodies in the rescue of FAP115 KO. (C) Swim speed measurements in wild-type (WT), FAP115 KO, and FAP115 rescue. (D) Duration of individual cilia power strokes measured in wild-type (WT), FAP115 KO, and FAP115 rescue strains based on images such as those shown in E. (E) Sample image of a Tetrahymena cell, showing identification of a single cilium (top) and single frames from time-lapse images of cilia power strokes measured in WT, FAP115 KO, and FAP115 rescue strains (bottom). *, p < 0.01; error bars = SEM.