FIGURE 2:

The abundance of nuclear TEAD is diminished in cells transfected with a ZO-2 construct lacking the NLS. (A) Representative image of parental MDCK cells 24 h after transfection with full-length Flag-hZO-2 (WT) or Flag-hZO-2 ΔNLS. Cells were fixed and processed for immunofluorescence with antibodies against Flag and TEAD. Nuclei were stained with DAPI. Arrows, nucleus of transfected cells. (B) Quantitative analysis of TEAD nuclear immunofluorescence in Flag-hZO-2 or Flag-hZO-2 ΔNLS transfected cells. The nuclei of 30 transfected cells per condition derived from three independent experiments were quantitated. AU, arbitrary units. Statistical analysis done with Student’s t test, *p < 0.05. Each bar shows the mean ± SEM.