FIGURE 4:

In ZO-2 KD cells inhibition of nPKCε increases the activity of a promoter regulated by TEAD-binding sites. Reporter gene assays done in parental and ZO-2 KD cells transiently transfected with hCTGF-Luc at a concentration of 100 ng/well (32 mm²). (A) hCTGF promoter activity is higher in ZO-2 KD than in parental cells. hCTGF promoter activity values were normalized to values observed in parental cells. Statistical analysis done with Student’s t test, *p < 0.05. (B) Inhibition of nPKCε increases the activity of hCTGF promoter in ZO-2 KD cells but not in parental cells. hCTGF promoter activity values were normalized to values observed in ZO-2 KD cells without the nPKCε inhibitor. Statistical analysis done with Student’s t test, *p < 0.05. In all cases data were normalized to protein content and transfection efficiency with pGL3-Control. Each bar shows the mean ± SEM from triplicates derived from two independent experiments.